Laboratory of Regulatory Systems Neuroscience
University of Pennsylvania
We perform stereotaxic surgery to  implant cannula for behavioral pharmacology  inject AAV-shRNA for viral mediated knockdown  inject cre-dependent AAV DREADDs for chemogenetic control of anatomically and phenotypically distinct neural populations.
Light and Florescence Microscopy
We do both light and fluorescence microscopy to histologically confirm surgical placements to chemically identify phenotypically distinct populations of neurons.
Meal Pattern Analysis
Animals are housed in a custom automated feedometer that has hanging wire bottom cages with an access hole to a food cup that rests on an electronic scale. The feedometer is connected to software that records the weight of the food cup every 10 seconds. Food spillage and body weight are manually measured. This allows us to collect meal size, meal number, inter meal interval and other meal pattern data.
Operant chambers are typically used for evaluation of appetitive and motivated feeding behaviors, where rats have to work to press a lever for palatable food.
Measurement of Energy Expenditure
Telemetric transponders are implanted into rats, which record and transmit correlates of energy expenditure including core temperature (TC), spontaneous physical activity (SPA), heart rate (HR).
Conditioned Place Prefernce
Conditioned Place Preference (CPP) is used to evaluate food seeking behavior when food is not available in the environment.